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DIAGENODE DIAGNOSTICS
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Hamamatsu
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Cytiva Europe
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Image Search Results
Journal: Protein Expression and Purification
Article Title: Structural characterization by transmission electron microscopy and immunoreactivity of recombinant Hendra virus nucleocapsid protein expressed and purified from Escherichia coli
doi: 10.1016/j.pep.2015.07.008
Figure Lengend Snippet: Size exclusion chromatography of HeV N constructs. Preparative scale size exclusion chromatography (SEC) purification of IMAC purified HeV N. (A) HeV N FL fractionated on Superdex 200 26/600 gel filtration column and (B) HeV N CORE fractionated on a Superdex 200 16/600 gel filtration column. Columns were run in 50 mM Tris, pH 7.5 containing 500 mM NaCl and 5% glycerol. The elution profile indicated a major peak with an apparent molecular weight of greater than 670 kDa, corresponding to high order oligmers of HeV N. The molecular weights (in kDa) of the gel filtration standards used for calibration are indicated at their integrated elution volume. The absorbance of the sample is measured at both A 280 and A 260.
Article Snippet: The concentrated protein was then fractionated by SEC using a
Techniques: Size-exclusion Chromatography, Construct, Purification, Filtration, Molecular Weight